Effects of domestic recombinant adenovirus p53 injection combined with cisplatin on growth and apoptosis of ovarian carcinoma cell lines
10.3781/j.issn.1000-7431.2009.07.006
- VernacularTitle:重组人p53腺病毒注射液联合顺铂对卵巢癌细胞生长及凋亡的影响
- Author:
Hongliang CHEN
;
Hong SUN
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Biological therapy;
Tumor suppressor protein p53;
Cisplatin
- From:
Tumor
2009;(7):631-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of domestic recombinant adenovirus p53 injection (rAd-p53,Gendicine) combined with cisplatin (DDP) on growth and apoptosis of ovarian carcinoma cell lines. Methods:MTT assay, flow cytometry, and Western blotting were used to compare the effects of rAd-p53, DDP and their combination on the growth, cell cycle, apoptotic rate, and p53 protein expression of ovarian carcinoma cell lines. Results:rAd-p53 inhibited ovarian carcinoma cells proliferation in dosE- and timE-dependent manners. After combined treatment, the growth inhibition rate was significantly increased (P<0.01). There was no significant diffE-rence in the inhibition potency induced by combined treatment with different sequences (P>0.05). Combined treatment significantly arrested ovarian carcinoma cell lines in G0/G1 phase at 72 h, the cell number in S phase was significantly decreased and the apototic rate was significantly increased (P<0.01). High expression of p53 protein was detected in ovarian carcinoma cell lines at 72 h after administeration of rAd-p53. Conclusion:rAd-p53 tranfects the exogenous widE-type p53 into the genome group of human ovarian tumor cells, induces p53 protein expression, thereby arresting tumor cells at G0/G1 phase, inhibiting tumor growth, and inducing apoptosis.
