Expression of ssrA in non-pathogen-induced adaptation in the oral cavity through signal exchange with oral pathogens.
10.5125/jkaoms.2012.38.1.14
- Author:
Sung Ryoul KIM
1
;
Jae Woo KWAK
;
Sung Ka LEE
;
Seung Gon JUNG
;
Man Seung HAN
;
Bang Sin KIM
;
Min Suk KOOK
;
Hee Kyun OH
;
Hong Ju PARK
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University, Gwangju, Korea. omspark@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
ssrA;
Escherichia coli;
Adaptation
- MeSH:
B-Lymphocytes;
Cell Line;
Escherichia coli;
Humans;
Mouth;
NF-kappa B;
Polymerase Chain Reaction;
Reverse Transcription;
RNA
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2012;38(1):14-19
- CountryRepublic of Korea
- Language:English
-
Abstract:
INTRODUCTION: This study was conducted to evaluate ssrA expression resulting from adaptation of Escherichia coli (E. coli) to oral pathogens through signal exchange. MATERIALS AND METHODS: Human cell lines Hep2 and HT29, wild-type E. coli (WT K-12), ssrA knock-out E. coli (Delta K-12), and Scleropages aureus (S. aureus) were used. A single culture consisting of Hep2, HT29, WT K-12, and Delta K-12, and mixed cultures consisting of Hep2 and WT K-12, Hep2 and Delta K-12, WT K-12 and S. aureus , Delta K-12 and S. aureus , and Hep2, WT K-12, and S. aureus were prepared. For HT29, a mixed culture was prepared with WT K-12 and with WT K-12 and S. aureus. Total RNA was extracted from each culture with the resulting expression of ssrA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB), and p53 was evaluated by Reverse transcription polymerase chain reaction (RT-PCR). RESULTS: The expression of ssrA in a single culture of WT K-12 was lower than that observed in the mixed culture of WT K-12 with S. aureus. Greater ssrA expression was observed in the mixed culture of WT K-12 with Hep2 than in the single culture of WT K-12. The expression of NF-kappaB was higher in the mixed culture of Hep2 with Delta K-12 than that in the mixed culture of Hep2 with WT K-12, and was lowest in the single culture of Hep2. The expression of ssrA was higher in the mixed culture of WT K-12 with Hep2 and S. aureus than in the mixed culture of WT K-12 with Hep2. CONCLUSION: These results suggest that ssrA plays an important role in the mechanism of E. coli adaptation to a new environment.
