A novel rapid fluorescence focus inhibition test for the detection of rabies virus antibody
10.3969/j.issn.1002-2694.2009.07.002
- VernacularTitle:狂犬病毒新型快速荧光斑点抑制实验方法的建立
- Author:
Hongru LIANG
;
Xiaohui LIU
;
Jing CHEN
;
Zhaojin SUN
;
Xiaofeng GUO
- Publication Type:Journal Article
- Keywords:
rabies virus;
green fluorescent protein;
rapid fluorescent focus inhibition test
- From:
Chinese Journal of Zoonoses
2009;(7):619-622
- CountryChina
- Language:Chinese
-
Abstract:
The objective of the present study was to establish a novel rapid fluorescence focus inhibition test (RFFIT GFP)for the detection of rabies virus antibody, in which a chimeric rabies virus expressing green fluorescent protein (HEP GFP) was used as the basic virus strain in RFFIT GFP assay, and a few serum samples from human, dog and cat were detected by this new method .The optimal serum dilution, virus dosage and infection time were determined in 24 serum samples from human, dog and cat by using RFFIT GFP, RFFIT and ELISA assays. The result showed that these 3 methods gave a good consistency. But RFFIT GFP was found to be more convenient and economic for the detection of rabies virus antibody.
