HPLC Determination of Isocorydine in JinshiMing Eye Gelatin
10.3969/j.issn.1005-9903.2009.07.005
- VernacularTitle:HPLC法测定近视明眼用凝胶中异紫堇定的含量
- Author:
Sen HUANG
;
Bo LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Jinshining Eye gelatin;
Isoeorydine
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2009;(7):12-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC method for determination of Isocorydine in Jinshining Eye gelatin. Method: AgiLentTC-C18 chromatographi coLumn(4.6 min×150 mm,5 μm) was used,methanoL-0.2% phosphoric acid soLution (adjust the pH 7.0 take methyL aLcohoL)(54:46)as the mobiLe phase, the detection waveLength was 267 nm,flow rate at 1.0 mL· min-1. Result: The calibration curve of Isocorydine as linear between 0.123 0～1.107 0μg, The average reeovery and the relative standard deviation were 97.11% and 1.65%. Conclusions: The method is simple, accurate, repeatable. It can be used for quality control of JinshiLe Eye gelatin.
