HPLC Determination of Saponins in Zhixiao Suppositories
10.3969/j.issn.1005-9903.2009.07.010
- VernacularTitle:痔消栓的HPLC含量测定
- Author:
Rong ZHANG
;
Xiuxia DENG
;
Gang WEI
;
Runmei LI
;
Wenzhou LI
;
Jun CHEN
- Publication Type:Journal Article
- Keywords:
Zhixiao suppositories;
notoginsenoside R1;
ginsenoside Rg1;
ginsenoside Rb1;
HPLC
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2009;(7):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To denvolop HPLC an method for the determination of the content of three saponins in Zhixiao suppositories. Methods: Analysis carriedout was performed on a Kromasil C18 column (4.6 mm×250 mm., 5μm)at 23℃. The mobile phases were methanol and 0.2% phosphoricacid solution in a gradient elution. The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 203 nm. Results: The linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.368～2.76μg,0.676～5.07 μg and 0.812～6.09μg,respectively. The average recoveries of the three saponins were 98.53%, 99.20% and 99.35%, respectively. The RSD (n=5) were 2.11%, 1.88% and 2.38%, respectively. Conclusion: The separation is a good and the results are accurate and reproducible. It can be used for the quality control of Zhixiao suppositories.
