The first identification and molecular epidemiology of PEC in China
10.3969/j.issn.1002-2694.2009.07.003
- VernacularTitle:首次从国内鉴定到猪肠道杯状病毒及其分子流行病学调查
- Author:
Zebin HUANG
;
Xinglong YU
;
Runcheng LI
;
Xiaoyu XIE
;
Deming YIN
;
Yunqiu YAN
;
Xia BAI
;
Zhonghua LIU
;
Jian DING
;
Zhennan WANG
;
Manxiang LI
- Publication Type:Journal Article
- Keywords:
porcine enteric caliciviruses;
porcine sapovirus;
diarrhea;
swine
- From:
Chinese Journal of Zoonoses
2009;(7):623-626
- CountryChina
- Language:Chinese
-
Abstract:
A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses (PEC) member porcine sapoviruses (SaV) by reverse transcription polymerase chain reaction (RT-PCR) amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% (24/189) of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related (75.6 88.3% identity) to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.
