Influence of OR-heparin,a chemically modified heparin,on high glucose-induced mesangial cell apoptosis and its signal transduction pathway
- VernacularTitle:氧化/还原修饰肝素对高糖诱导肾小球系膜细胞凋亡及其信号转导途径的影响
- Author:
Dengke YIN
;
Wenbing YAO
;
Xiangdong GAO
- Publication Type:Journal Article
- Keywords:
OR-heparin;
mesangial cell;
apoptosis;
high glucose
- From:
Journal of China Pharmaceutical University
2008;(4):358-364
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether chemically modified non-anticoagulation heparin derivate (periodate oxidation/borohydride reduction-modified heparin (OR-heparin)) could inhibit high glucose-induced human mesangial cell apoptosis and to explore its possible mechanism.Methods:Mesangial cells were exposed to high glucose or high glucose with OR-heparin for 24 h.Apoptosis was evaluated by Hoechst 33258 staining and Fluorescent activated cell sorting analysis.The expressions of apoptosis-related proteins ( p53,Bcl-2,Bax,cytosolic cytochrome C) were determined by Western blotting.NF-kB translocation was observed under fluorescence microscopy by using Cy3-1abeled antibody.Caspase-3 activity was detected by colorimetry.Results:OR-heparin significantly inhibited high glucose-induced mesangial cell apoptosis and the expression of apoptosis-related proteins.It also blocked NF-kB translocation induced by high glucose.Conclusion:OR-heparin inhibits high glucose-induced mesangial cell apoptosis through inhibiting the expression of apoptosis-related proteins and it may be due to the blockage of the translocation of NF-kB.
