IMMUNE RESPONSES OF MICE VACCINATED WITH RECOMBINANTS CODED DIFFERENT STAGE GENES FROM FCC 1/HN STRAIN OF PLASMODIUM FALCIPARUM
10.3969/j.issn.1002-2694.2000.04.009
- VernacularTitle:恶性疟原虫FCC-1／UN株不同期基因重组质粒混合接种小鼠后的免疫反应
- Author:
Fangli LU
;
Xinbing YU
;
Haifeng CHEN
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum;
strain;
pcDNA3-EBA175/HRP Ⅱ;
pcDNA3-Pfs25;
Immuneresponse
- From:
Chinese Journal of Zoonoses
2000;(4):28-32
- CountryChina
- Language:Chinese
-
Abstract:
Aim To search the effective malaria mixed gene vaccine and to explore the immune response of vaccinated host. The recombinant pcDNA3-EBA175/HRP Ⅱ was injected alone or mixed with pcDNA3-Pfs25 of Plasmodium falciparum into mice by intramuscular route. The muscle of injected parts were treated previously, e.g. injecting 50μl of 0. 5％bupivacaine into the muscle of left latter leg, with 2mm depth. To observed the changes of IgG antibody value, the splenic lymphocyte proliferation, the ratio of CD4+/CD8+ subgroups and NK cell killing activity. After injecting recombinant pcDNA3-EBA175/HRP Ⅱ alone or mixed with pcDNA3-Pfs25 into mice by intramuscular route, it showed that sera IgG value increased, the splenic T lymphocyte proliferation stimulated by specific antigen of Plasmodium falciparum increased, the ratio of CD4+/CD8+ decreased and NK cell activity increased. Enhanced immune injection could improve host's immune reaction.It is suggested intramuscular injection is an effective immune route, and mice inoculated with coding two gene recombinant alone or that mixed with sexual stage gene recombinant could all induce increased humoral and cellular immune response, and NK cell activity.
