DETECTION OF MYCOPLASMA FERMENTAN AND MYCOPLASMA PENETVOM BY DUAL NESTED - PCR
10.3969/j.issn.1002-2694.2000.04.016
- VernacularTitle:发酵支原体、穿通支原体双重套式PCR检测研究
- Author:
Zuhuang MI
;
Ling QIN
- Publication Type:Journal Article
- Keywords:
Mycoplasma fermentan;
Mycoplasma penetran;
Dualnested-PCR
- From:
Chinese Journal of Zoonoses
2000;(4):50-52
- CountryChina
- Language:Chinese
-
Abstract:
Aim In order to detect Mycoplasma fermentans (Mr)and Mycoplasma penetrans (Mpe) at the same time dual nested-PCR(DN-PCR) technique was established Method Mpe the same time PCR Primers were designed according to published 16s rRNA Sequences of Mf and Mpe, the outer primers were univevsal to both Mf and Mpe and the inner was Mf、Mpe speciespecific respectively. Two-step amplification procedures were perfomed. Result Chromo some DNAS of Mf and Mpe gave characteristic DN-PCR prafiles which were differeht from other my coplasmas or microorganism tested.Conclusion The DN-PCR method was established for the detection of Mf and Mpe at the same time which was sensitive specific and rapid.
