PREPARATION AND APPLICATION OF HUMAN PRP SYNTHYETIC PEPTIDE
10.3969/j.issn.1002-2694.2000.04.005
- VernacularTitle:人类PrP合成肽抗原构建及应用
- Author:
Songyan LIU
;
Shihe LIN
;
Shuling LIU
- Publication Type:Journal Article
- Keywords:
PrP synthetic peptide;
Bovine serum albumin;
Counled
- From:
Chinese Journal of Zoonoses
2000;(4):15-17
- CountryChina
- Language:Chinese
-
Abstract:
Aim In order to improver the diagnostic rate of Prion disease, solve the lacking sources of nature PrP antigen. ,synthesize the human PrP peptide, prepare the antibody to PrP peptide further. Method A synthetic peptide with the sequence identical to the 15 residues of human PrP, as described by Kretzschmar[1], was synthesized by the solid-phase method. The synthetic peptide was coupled to bovine serum albumin(BSA)by the method of EDCI〔2〕. The polypeptide combined with BSA was used as antigen to immunize the rabbit and detected by immunomethod. Result The PrP polypeptide combined with BSA obtained immunogenicity and anti-PrP synthetic peptide antiserum was successfully obtained. Conclusion The preparation and application of human PrP synthetic peptide can substitute for nature PrP antigen partly. It has laid a foundation for further preparation of monoclonal antibody to PrP and the study of Prion disease.
