STUDIES ON IMMUNO-RESPONSE OF DNA VACCINATION WITH RECOMBINANT PLASMID pcDNA3 CONTAINING ROP1 GENE FROM TOXOPLASMA GONDII IN BALB/C MICE IV. The Detecting of IFN-γ,IL-2,and NO in the Serum from the Immunized Mice
10.3969/j.issn.1002-2694.2000.04.006
- VernacularTitle:含弓形虫ROP1基因真核表达重组质粒DNA免疫小鼠的研究*IV.免疫鼠血清细胞因子IFN-γ、IL-2及NO的测定
- Author:
Hong GUO
;
Guanjin CHEN
;
Huanqin ZHENG
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Rhoptry protein1 (ROP1);
DNA vaccination;
Recombinant plasmid
- From:
Chinese Journal of Zoonoses
2000;(4):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Aim DNA vaccinating BALB/c mice with the constructed recombinant plasmid, pcDNA3, containing ROP1 gene from Toxoplasma gondii to observe the effect on the production of the cytokines, IFN- γ、 IL - 2 , and NO in the immunized mice. Methods Large-scale preparation of plasmid DNA by alkaline lysis,the DNA were injected through muscles of left leg in each mouse at the dosage of 100μg. A booster vaccine was given at the same dosage after two weeks. Control groups were injected with pcDNA3 blank plasmid and normal saline respectively. After 30,50 and 70 days of the booster injection, following tests were carried out 3 times separately :the serum IFN-γand IL-2 were detected by sandwich ELISA;the NO was detected by enzyme assay. Results The 3 times detected results of IFN-γγ、IL-2 and NO were significant higher in the vaccinated group than that of in control groups and the contents were increased with the vaccinated time prolonged. Conclusion The IFN - γγ、 IL - 2 and NO were produced by vaccinating BALB/c mice with the recombinant plasmid, pcROP1.
