Isolation of 9 HIV-1 strains from the Infected Persons and Study of Their Biological Characteation
10.3969/j.issn.1002-2694.2000.02.002
- VernacularTitle:9株艾滋病病毒的分离及生物学特性的研究
- Author:
Yansheng YAN
;
Huirong WANG
;
Ge CHEN
;
Aihua HE
- Publication Type:Journal Article
- Keywords:
Human Immunodeficiency Vinus tyoe 1: Isolation: HIV-1 cell tropism
- From:
Chinese Journal of Zoonoses
2000;(2):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Aim To isolate HIV-1 from the blood specimen of HIV-infected persons, and study on the features of the isolates. Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively. Results HIV-1 was isolated from9 of the 10 specimens, the isolation rate could reached 90%. The 9 isolates were used to infect MT4 cells, respectively, and all of them could cause cell syncytias.8 of the isolates showed only a transient infection in MT4 cells, but the isolate FJ113 could infect MT4 successively, OD values kept no changes even it passed 15 generations, therefore, it is consider as a rapid/high replicating virus. Conclusions Addition of neuramindase into T cell culture medium may useful to raise HIV isolation rate. Most of HIV-1 isolates in Fujian are M tropic.
