STUDY ON DETECTION OF HEMORRHAGIC FEVER WITH RENAL SYDNROME VIRUS GENE BY DIG LABELED PROBE
10.3969/j.issn.1002-2694.2000.02.004
- VernacularTitle:地高辛素标记探针检测HFRS病毒基因研究
- Author:
Jin ZHU
;
Yun ZHANG
;
Hengbin GUO
;
Jiaqi TANG
;
Gusnghua WU
- Publication Type:Journal Article
- Keywords:
Dot-blot hybridization Chigger mite Hemorrhagic fever with renal syndrome Virus
- From:
Chinese Journal of Zoonoses
2000;(2):13-15
- CountryChina
- Language:Chinese
-
Abstract:
Dig-DNA probes of hemorrhagic fever with renal syndrome vinus M S gene fragment were prepared by PCR and random primer labeling, The probes had speciality to HFRSV-RNA and could detect 10pg RNA by RNA-DNA dot-blot hybridization. The results of hybridization showed that positive reactions were found in 5, 10 chigger mites groups from antigenic positive rats, 10 mites group from antigenic negative rats, 10, 50 free chigger mites, and hung of antigenic positive mice 100mg, 500mg. But negative reation in 5 mites group from antigenic negative mice.
