THE FIELD TRIAL ON RAPID DETFCTION OF SCHISTOSOMA CIRCULATING ANTIGENS WITH DOT IMMUNOGOLD FILTRATION ASSAY (DIGFA)
10.3969/j.issn.1002-2694.2000.02.006
- VernacularTitle:金标渗滤法快速检测血吸虫循环抗原的现场应用研究
- Author:
Xiaoxian GAN
;
Liying SHEN
;
Jianzu DING
;
Huiying SHEN
- Publication Type:Journal Article
- Keywords:
Schistosomiasis;
DIGFA;
Circulatiug antigen;
Rapid detection
- From:
Chinese Journal of Zoonoses
2000;(2):19-20
- CountryChina
- Language:Chinese
-
Abstract:
A total number of 2221sera collected from different kinds of subjects , i.e.patients with various stages of schistosomiasis. normal individuals, persons with other parasitic diseases or non-parasitic diseases, were detected with Dot Immunogold Filtration Assay (DIGFA) using anti-SVLBP IgG as capturig antibody. Positive rate of 70 sera from patients with acute schistosomiasis was 100%, and that of 307 sera with chronic schistosomiasis was 68.4% .None of 200 sera from normal individuals showed false positive reaction. No obvious cross reaction was found in sera from other parasitic/non-parasitic diseases except sera from patients with paragonimiasis.2 batches of sera were detected with DIGFA by single-blindness method. Results showed that sensitivities to chronic schistosomiasis were 69.4% and 68.9%, and 100% to acute schistosomiasis while specificities were 98.9%-99% .508 samples from residents in endemic areas were tested with DIGFA and Kato-Katz. Positive rate of circulating arrtigen was 20.9% with DIGFA while 65 samples were positive with stool examination.46 samples were positive both by DIGFA and by Kato-katz.The coincidental rate was 70.8% .These results indicated that DIGFA showed sensitive and specific in the detection of circulating antigen. It will be useful in mass application and vahuable in epidemiological stuvey.
