ANALYZING CLINICAL SAMPLES OF LYME DISEASE WITH 5S-23S RRNA GENE SPACER RFLP ANALYSIS
10.3969/j.issn.1002-2694.2000.02.007
- VernacularTitle:5s-23sRAN基因间隔区RELP分析对莱姆病病例尿标本的检测
- Author:
Cuixia SHI
;
Zhefu ZHANG
;
Kanglin WAN
- Publication Type:Journal Article
- Keywords:
5s-23srRNA genespacer RFLP analysis;
genospecies;
Lyme disease
- From:
Chinese Journal of Zoonoses
2000;(2):21-23
- CountryChina
- Language:Chinese
-
Abstract:
The author tested 34 urine samples with 5s-23srRNA gene spacer RFLP analysis and compared PCR results with serodiagnosis results.8 pafients of urine are positive by PCR. 7 patients were infected by B. garinii, 1 patient by B afzelii. These genospecies infecting patients are identical with those infecting local vectors. The results showed that the accuracy of laboratory diagnosis oould be improved by simultaneously testing samples with PCR and serodiagnosis. The method of 5s-23srRNA gene spacer RFLP analysis will probably be used widely in studying the relationship of clinical manifestation and genospecies and in epidemiology investigations.
