T CELL IMMUNE RESPONSE TO THE CIRCUMSPOROZOITE PROTEINOF PLASMODIUM FALCIPARUM, SOUTHERN CHINA ISOLATE FCC/HN AND ANTIGEN-INDUCED HL-2 AND IFN-γ Production ANALYSIS OF SPECIFIC

VernacularTitle:恶性疟原虫FCC1／HN株CSP抗原细胞免疫应答IL-2和IFN-γ的体外诱生分析
Author: Zheng FANG ; Yanwen LIU ; Weiqun HUANG ; Xin JI ; Yukun SHI ; Xinbing YU
Publication Type:Journal Article
Keywords: Plasmodium falciparum; Circumsporozoite antigen; Cell immune response; IL-2; IFN-γ
From: Chinese Journal of Zoonoses 2000;(2):32-34
CountryChina
Language:Chinese
Abstract: Inthis study, the DNA fragment encoding the regions Ⅰ to Ⅱ of CSP gene from Plasmodium falciparm isolate FCC1/HN was cloned into an. expression vector pcDNA3 contained cytomegalovius (CMV) and transformed into human Hela cell line. The expressed protein PfCSP (condidate vaccine) was used to immunize BALB/c mice by subcutaneous、 intravenous or intraperitoneal administration respectively. The splenocyte of BALB/c mice immumized with the condidate vaccine released significantly IL-2 and IFN-γ following stimulation with this vaccine. It is associated with increase of the splenic T lymphocyte proliferation stimulated by this vaccine and enhancement of NK cell killing activity in the former studies. These results suggested that the vaccine could stimulate T cell response and enhance the cell-mediated immunity.