IN SITU BY BRIDIZATION DETECTING L- FORM DNA OF STAPHYLOCOCCUS AUREUS OF LARYNGOCARCINOMA
10.3969/j.issn.1002-2694.2000.02.016
- VernacularTitle:原位杂交检测喉癌中金葡菌L型DNA
- Author:
Shuxiu XU
;
Wanying WANG
;
Ming YAO
- Publication Type:Journal Article
- Keywords:
Laryngocarcinoma: L-form Staphylococcus aureus;
Gram staining;
Immunohistochemical;
Nucleic acid in situ hybridization
- From:
Chinese Journal of Zoonoses
2000;(2):54-55
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study whetger there is positive express in situ hybridization of L-form DNA or Staphylococcus aureus in laryngocarcinoma cell. Methods 20 cases of expressnon of L-form DNA of Staphylococcus aureus in laryngocarcinoma karyon were detected with nucleic acid in situ hybridization. Results 60% cancer karyon, cytoplasm display positive signal of Staphylococcus aureus L-form DNA. Positive rate of Staphylococcus aureus L-form antigen is 75% with immunohischemical (s-p) stainipg. The rate is 70% with gram staining. Conclusion Staphylococcus aureus L-form DNA had get into laryngocarcinoma ceells, is great possibly to integrate into host ce11, and influence hyperplasis and canceration of cell from gene level. Staphylococcus aureus and its L-form infection have possibly close relation to happen and development of laryngocarcinoma.
