STUDY ON CHANGES OF T LYMPHOCYTE SUBSETS IN MICE IMMUNIZEDBY RECOMBINANT BCG-SJ26GST VACCINE AGAINST SCHISTOSOMA JAPONICUM
10.3969/j.issn.1002-2694.2000.02.017
- VernacularTitle:血吸虫重组BCG-Sj26GST疫苗诱导小鼠T淋巴细胞亚群变化的研究
- Author:
Wengui LI
;
Youen SHI
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
BCG-Sj26GST vaccine;
phenotype of lymphocyte;
Mice
- From:
Chinese Journal of Zoonoses
2000;(2):56-58
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects on T lymphocyte subsets in spleen of mice immunized with recombinant BCGSj26GST. Methods Inthe first experiment, BALB/c mice were immrnized subcutaneously by 106 and 108 CFU BCG-Sj26GST respectively. ALLthe mice were artifically challenged with cerariae of Schistosoma japonicum on 8weeks after immunization, six after challenge, the mice were killed, and the spleens were removed, cells were prepared seperately and were analysed by immunofluorescence with conjugated monoclonal antibodies in a FACsort cytofiuorimeter at 523 nm, PBS treated mice were serwed as control. In the second experiment, after immunized subcutanenously or inntraveously by 106 CFU vaccine, 4 mice were ranndonly killed to separate spleens on 0wk, 4wk, 8wk, 10wk, 14wk and 16wk after immurization, the percentage of CD+4 and CDs+ subsets was analysed as above. Results In the first experiment, CD+4 subsets increased renarkably, but CD+8 subsetsdid slightly by immunization with the vaccine against challenge with S.j. Cercariae, in the second experiment, the dynamic observation showed that in the subcutaneous and intravenous group, CD+4 subsets increased obviously since 10wk and 8wk respectively, CD8+ subsets hadno obvious changeover 16 weeks of observation , in the subcutaneous groupbuttheCD8+ subsets rose lightly on 4-16wk in the intravenous group. Conclusion On the basis of this study it's suspected that CD+4 subsets might play an important role in the protective immunity induced by the vaccine
