ISOLATION OF FEMALE－ SPECIFIC SEQUENCE OF SCHISTOSOMA JAPONICUM
10.3969/j.issn.1002-2694.2000.02.023
- VernacularTitle:代表差异性分析方法分离日本血吸虫雌虫特异性序列
- Author:
Yi WANG
;
Ximei ZHAN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum RDA DNA hybridization
- From:
Chinese Journal of Zoonoses
2000;(2):72-73
- CountryChina
- Language:Chinese
-
Abstract:
Aim To isolate the female-specific sequence of Schistosomajaponicum S. J. in order to provide a basis for researching vaccines of anti-fecundity. Methods Representational difference analysis (RDA) was used to obtain a female-specific sequence of S.j. by the male S.j. genomic DNA substractive hybridization with the female. The fragment was about 562bp. Results This sequence was labeled as Dig probe by PCR technique to hybridize with the restriction fragments of each sex and the tester as well as the driver on the nylon membranes.The antiboly of Dig、 NBT、 and X-phosphate solution were utilized to immunoassay the result of hybridization. The result showed that the probe can hybridize to the tester but can not hybridize to the driver. Conclusion The result suggested that the fragment of approx. 562bp was the female-specific sequence of Schistosoma japonicum.
