DETECTION OF CHLAMYDIA PNEUMONIAEBY NESTED POLYMERASE CHAIN REACTION
10.3969/j.issn.1002-2694.2000.02.027
- VernacularTitle:肺炎衣原体套式聚合酶链式反应检测研究
- Author:
Ling QIN
;
Zuhuang MI
;
Yahua LU
- Publication Type:Journal Article
- Keywords:
Chlamydia pneumoniae;
Nested polynerase chain reaction
- From:
Chinese Journal of Zoonoses
2000;(2):84-86
- CountryChina
- Language:Chinese
-
Abstract:
Aim To develop a molecular biologic technique for detection of chlamudia pneumoniae with nested polymeyase chain reaction (nPCR) .Methods Nested primers were synthesized according to a cloned C.pneumoniae 474- bp Pst I fragmert. Results The 378bp DNA fragments were amplified from C. pneuomoniae with nPCR. None of the C. trachomatis, C. psittaci, other organisms and etc. Strains tested were amplified by the nPCR. The direct sequening of 3 sample products with nPCR are quite same as C. pneumoniae (CWL-29 ) . The sensitivity of nPCR is higter than PCR. Conclusions This method is not only sensitive.specific and rapid, but also provides an etiological basis for cdiagnosis of C.pneumoniae infection.
