AMPLIFICATION, CLONING AND PROKARYOTIC EXPRESSION OF GENE FOR MAJOR SURFACE ANTIGEN OF TOXOPLASMA GONDII
10.3969/j.issn.1002-2694.2000.02.003
- VernacularTitle:弓形虫主要表面抗原基因编码序列的扩增、克隆及原核融合表达
- Author:
Dongsheng YOU
;
Jilong SHEN
;
Hua MA
;
Keshen DAI
;
Long YU
- Publication Type:Journal Article
- Keywords:
T. gondii;
P30;
Cloning;
GST-fused expression;
Western blot
- From:
Chinese Journal of Zoonoses
2000;(2):9-12
- CountryChina
- Language:Chinese
-
Abstract:
Aim To amplify P30 gene and express P30 fusion with GST Methods P30 gene was smplified from T. gondii chromosomal DNA and ligated to pGEM-T and pGEX-4T-1. Screening-positive recombinants were induced for expression, which was subsequently detected by WB Results P30 gene was amplified and GST-fusion was confirmed by rabbit antiT. gondii serum. Conclusions The construction of pGEM-T-P30 and pGEX-4T-1-P30, together with the recombinant protein would lay a base for further investigation of P30 at a molecule-level and application to diagnosis and vaccination
