Rapid quantification of tiopronin in human plasma by high-performance liquid chromatography-mass spectrometry
- VernacularTitle:HPLC-MS法快速定量分析人血浆中硫普罗宁浓度
- Author:
Na LIU
;
Beibei ZHANG
;
Zunjian ZHANG
;
Yuan TIAN
;
Fengguo XU
;
Yun CHEN
- Publication Type:Journal Article
- Keywords:
tiopronin;
cysteine;
HPLC-MS;
bioequivalence
- From:
Journal of China Pharmaceutical University
2008;(1):46-51
- CountryChina
- Language:Chinese
-
Abstract:
Aim:To develop a rapid high-performance liquid chromatography-mass spectrometry (HPLC-MS) method for the quantification of tiopronin in human plasma.Methods:Cysteine was chosen as antioxidant and firstly added into the whole blood firstly.After adding mycophenolic acid as internal standard (IS) and 1 mol/L HCl into the plasma,the samples were extracted with acetic ether and then determined by HPLC-MS.The chromatographic separation was performed on a Shim-pack VP-ODS C18 column (250 mm×2.0 mm,5 μm) using methanol-0.1% acetic acid (70∶30) as mobile phase with a flow rate of 0.2 mL/min.Results:The assay exhibited a linear range of tiopronin between 30～4 000 ng/mL.The precisions for intra- and inter-batch were all within 8.5%.The extraction recoveries were more than 70%.The total HPLC-MS analysis time was within 7.5 min per a run.The fully validated method was successfully applied to quantify tiopronin in human plasma for a bioavailability study.Conclusion:The assay proved to be accurate,sensitive,selective and convenient.The fully validated method can be applied to study the pharmacokinetics and bioavailability of tiopronin and tiopronin formulations in human.
