Neoglycoprotein preparation of YCP, a polysaccharide from Phoma herbarum YS4108 and immunogenicity evaluation in mice
- VernacularTitle:海洋真菌多糖YCP的拟糖蛋白制备及其免疫原性比较
- Author:
Hu ZHAO
;
Hua ZHANG
;
Ying LU
;
Xiangdong GAO
- Publication Type:Journal Article
- Keywords:
polysaccharide;
YCP;
neoglycoprotein;
immunogenicity;
isotype
- From:
Journal of China Pharmaceutical University
2006;(4):361-366
- CountryChina
- Language:Chinese
-
Abstract:
Aim:To characterize the immune response to YCP, a polysaccharide (PS) isolated from marine filamentous fungus Phoma herbarum YS4108. Methods:YCP was coupled to bovine serum albumin (BSA) to construct three neoglycoproteins which are different in their degrees of substitution (DSs; ≈ 3, ≈ 6, or ≈ 10 mol of BSA carrier/mol of YCP hapten, respectively), and their immunogenicities were evaluated in mice following subcutaneous immunization,respectively. IgG subclasses against the PS and the carrier protein were measured in addition to the total IgG and IgM antibodies for YCP induced by the neoglycoproteins. Results: While unconjugated PS was weakly immunogenic, theneoglycoprotein induced vigorous primary IgM and booster IgG responses to PS and the carrier protein. Interestingly,the IgG subclass distribution was different between PS and the carrier protein; for PS, the IgG response was predominant of IgG2a subclass with approximately low levels of IgG2b and IgG1, while the response to the carrier protein was mainly of the IgG1 subclass with relatively low levels of IgG2a and IgG2b, and the lowest of IgG3. Conclusion:YCP has the potential to elicit preferentially IgG2a as the dominant isotype antibody in mice.
