Humoral and cellular immune responses induced by DNA vaccine based on major outer membrane protein of chlamydia trachomatis
10.3969/j.issn.1000-8861.2005.06.007
- VernacularTitle:沙眼衣原体主要外膜蛋白核酸疫苗诱导小鼠产生特异性细胞和体液免疫应答
- Author:
Zhongyu LI
;
Yimou WU
;
Minjun YU
;
Chaoqun CHEN
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
DNA vaccine;
Humoral immune response;
Cellular immune response
- From:
Immunological Journal
2005;(6):457-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct DNA vaccine containing MOMP gene of Chlamydia trachomatis and to observe immune response in mice. Methods Mice of 4 - 6 weeks old were immunized with pcDNA3.1-MOMP or pcDNA3.1 intramuscularly at a dose of 100 μg. Booster immunizations were employed at 2-week interval for two times. Specific antibody in the sera of mice and the level of IFN-γ in murine spleen lymphocyte supernatant were detected by ELISA. The proliferation response of spleen cells was detected by MTT assay. Results Significant specific antibody titers were observed and the highest titer was 1: 1 024 in mice after three times immunization with pcDNA3.1-MOMP. The proli-feration response of spleen cells were significantly higher than that of mice injected with pc DNA3.1. IFN-γ reached(532.0 + 45.4)pg/mL in immunized mice. Conclusion Strong responses of humoral and cellular immunity can be evoked by DNA vaccine of pcDNA3.1-MOMP in mice.
