Different frequencies of B lymphocytes committed to produce anti-thyrotropin receptor antibodies in patients at different clinical stages of Graves' disease
10.3969/j.issn.1000-8861.2002.01.001
- VernacularTitle:不同临床期Graves'病患者外周血分泌TRAb前体细胞频率的差异
- Author:
Weiguo JIA
;
Decai CHEN
;
Zhenmei AN
;
Songquan WEI
- Publication Type:Journal Article
- Keywords:
Graves' disease;
thyrotropin receptor;
autoantibody;
B lymphocyte;
precursor
- From:
Immunological Journal
2002;(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the TRAb-producing cell precursors and different clinical stages of Graves' disease.Methods Peripheral lymphocytes were infected with Epstein-Barr virus,a kind of lymphocyte precursor stimulator,and were stimulated to produce TRAb.Results Of the total wells containing Ig-producing B cell precursors,there were 34.6% and 29.1% from 2 untreated patients,30% from 1 relapsing patient and only 1.3% and 3.8% from 2 healthy controls that secreted TRAb,respectively.As for the wells containing B cell precursors from 2 remission patients with undetectable TRAb in circulation,the percentage (10.7%) of TRAb-containing wells from one was higher than that from the other (5.3%).And the latter had similar percentage of TRAb-positive wells with the controls.The isotype of TRAb in supernatants of our EBV-transformed B lymphocytes was predominantly IgM.Conclusion The frequencies of TRAb-specific precursors were different at three distinct clinical stages of the disease.
