Determination of the point of gene mutation in two types of tissue from the aminoglycoside antibiotics induced deaf patients
10.3969/j.issn.1001-1781.2001.04.002
- VernacularTitle:氨基甙类抗生素中毒性耳聋患者头发和血液的基因突变位点检测
- Author:
Lin XU
;
Weiwei CHEN
;
Changquan XING
- Publication Type:Journal Article
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2001;(4):149-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To elucidate the gene mutation presents in hair follicle cells,just as blood cells,in the aminoglycoside antibiotics induced deaf patients (AAID). Method:Mitochondrial DNA of blood cells and/or hair follicle cells from 8 members of 3 aminoglycoside antibiotics induced deafness families were analysed using PCRSSCP and Alw 26 Ⅰ digestian. Result:Our result showed that a gene mutation at nuleotide 1555 in 12 S rRNA of mitochondrial DNA appeared in blood cells and/or hair follicle cells of 7 subjects among those 8 members. Only a father with normal hearing did＇t exhibit such a mutation. Conclusion:This indicated that hairs could be used as a sample instead of blood to perform gene examination for AAID.
