Determination of racecadotril and its impurities by HPLC
- VernacularTitle:HPLC测定消旋卡多曲的含量及杂质
- Author:
Zhe ZHAO
;
Shuchun LIU
- Publication Type:Journal Article
- From:
Chinese Pharmaceutical Journal
2001;(4):267-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE A HPLC method was established for the determination of racecadotril and its impurities.METHODS A ODS column was used and the mixture of acetonitrile-potassium dihydrogen phosphate (KH2PO4) solution (70∶30) was used as the mobile phase.The detection wavelength was 210 nm.RESULTS The liner range of the racecadotril was 0.08～0.24 mg*mL-1 and the regression equation was Y=15847X+3873(r=0.9997).The liner range of benzylthiorphan disulphide was 2.40～21.56 μg*mL-1 and the regression equation was Y=1826x+46 (r=0.9999).The measurable lowest limit was 1 μg*mL-1.The average recovery was 100.0%.CONCLUSION The method was convenient,accurate and specific.
