Determination of uric dopamine content by HPLC-RF for patients with Parkinsons disease treated with laser acupuncture
- VernacularTitle:HPLC-RF法测定激光针刺治疗帕金森病患者尿液中多巴胺浓度
- Author:
Cangsang SONG
;
Shan LIN
;
Peilin ZHANG
;
Qin ZHANG
;
Zhongkun LI
;
Renqiu LI
- Publication Type:Journal Article
- From:
Chinese Pharmaceutical Journal
2001;(4):269-271
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To determine the concentrations of dopamine (DA) in human urine,studying the change of uric dopamine content before and after laser acupuncture treated Parkinsons disease patients.To estabilsh attemptly the standard of apprasing the treatment method which is combined traditional medical theory with modern technology.METHODS The urine samples were extracted with organic solvent and determined by HPLC-RF.RESULTS A good correlation was obtained between dopamine over 20～400 ng*mL-1 and peak height,r=0.9996.The recoveries of DA were (89.69±3.95)%～(92.99±6.5)% for urine samples.There was a close correlation between DA content and the severity of parkinsons disease.The DA content in urine of patients before acupuncture was less than healthy ones.DA content in urine increased most at 4 h and content still maintained high as normal scope at 12 after acupuncture.CONCLUSION This HPLC-RF assay was sensitive,accurate stable and convenient.It is an important method to evaluate the effects of treated Parkinsons disease by laser acupunctrue.
