Separation of Ephedrine Isomers with Non-aqueous Capillary Electrophoresis

VernacularTitle:非水系统毛细管电泳分离麻黄碱类差向异构体
Author: Yibing JI ; Yuying CHEN ; Rujin WU
Publication Type:Journal Article
From: Journal of China Pharmaceutical University 2001;(3):213-216
CountryChina
Language:Chinese
Abstract: AIM　Here the development of a simple, rapid and simultaneous method for the separation of five ephedrine alkaloids obtained with non-aqueous capillary electrophoresis (NACE) were reported. The effects of the electrolyte, non-aqueous solvent on the separation were also discussed. METHODS　A running buffer of 50 mmol/L ammonium acetate in methanol was found to be the most suitable for this separation. RESULT　The best separation was achieved within 8 minutes. CONCLUSION　The separation is not only dependent on differences in the pK value of the alkaloids but also on the intramolecular interaction and solvation. So much improved selectivity could be achieved in nonaqueous medium.