Influences on Serum Insulin and Glucose by Different Fasting Time in Alloxan Diabetic Mice
- VernacularTitle:不同禁食时间对四氧嘧啶糖尿病小鼠模型的血清胰岛素和血糖的影响
- Author:
Min HUANG
;
Shukui WANG
;
Xiaofeng WANG
;
Dezai DAI
;
Zizheng WANG
- Publication Type:Journal Article
- From:
Journal of China Pharmaceutical University
2001;(3):217-220
- CountryChina
- Language:Chinese
-
Abstract:
AIM The purpose is to study the influences on serum insulin and glucose by different fasting time in alloxan (ALX)-induced diabetic mice. METHODS Normal mice of serum glucose were determined at different fasting time. According to different fasting time (0 h, 6 h, 12 h, 18 h and 24 h), mice were divided into 5 diabetic experimental model (DEM) groups induced by ip ALX 200 mg/kg and 1 control group. Serum glucose (SG) and body weight were determined at experimental d0, d3, d8 and d13 and serum insulin concentration (SIC) at experimental d13. The death rate and succeeding rate were also observed during experimental time. RESULTS Along with prolonged fasting time, SG、death rate and succeeding rate were increased in DEM mice, SG of normal mice, and SIC and body weight of DEM mice were decreased. Results showed that the optimal fasting time of ALX-induced diabetic mice was 12～18 h a lower death rate and impairment to pancreatic islets. Fasting 18h diabetic mice had higher succeeding since during this time rate、lower death rate and longer hyperglycemia time. CONCLUSION This study indicated SG of mice in vivo had a protective effect against impairment action of pancreatic islets by ALX.
