Establishment of method of cloning alloantigen-specific T lymphocyte
10.3969/j.issn.1000-1492.2001.02.009
- VernacularTitle:同种抗原特异性T淋巴细胞克隆方法的建立
- Author:
Gongyao TANG
;
Jun QIAO
;
Wushu ZHAO
- Publication Type:Journal Article
- From:
Acta Universitatis Medicinalis Anhui
2001;(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of alloantigen-specific T lymphocyte clones. Methods PBMC of the recipient was stimulated by donor splenocytes for twice and the stimulated T lymphocytes were cloned by limited dilution. Results Three clones of T lymphocytes were obtained. By analysis of immunohistochemistry, two of the three clones were CD3+CD4-CD8+, another one was CD3+CD4-CD8-. The latter was analyzed by flowcytometry and CD3+ was 98.87%, CD4- 97.05%, CD8- 97.14%. Antigen specificity of the cloned T lymphocytes was determined by stimulation of donor splenocytes and non-related individual splenocytes. When stimulated by donor splenocytes, the cloned T lymphocytes proliferated and did not show proliferative role when stimulated by non-related individual splenocytes. Conclusion Our method of cloning alloantigen-specific T lymphocytes is successful.
