Study on susceptibility test of pathogenic fungi from otorhinolaryngology by Etest method
10.3969/j.issn.1001-1781.2001.02.012
- VernacularTitle:Etest法对致病真菌的药物敏感性检测
- Author:
Jushang LI
;
Li CHENG
;
Huitu NONG
;
Dongxiao NONG
- Publication Type:Journal Article
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2001;(2):77-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the sensitivity of pathogenic fungi from otorhinolaryngology to 5 antifungal agents of Ketoconazole(KE), Itraconazole(IT),Fluorocytosine(FC),Amphtericin B (AP) and Fluconazole(FL) by Etest method.Method:Etest for determine MIC of 14 pathogenic fungi was performed according to the manufactuer′s instructions.The Etest strip containing the 5 antifungal drugs were putted on medium with Candida albicans,A.flarus and A.fumigatus etc,respectively,to determine MIC of antifungal drugs.Result:Candida albicans were sensitized to all the above 5 antifungal drugs, KE and IT were most sensitized.Seven fungi such as A. flavus, A fumigatus, A.oryzae etc were all sensitized to KE and IT.The MIC range of KE and IT against 15 strains of pathogenic fungi were≤0.008～2 mg/L and ≤ 0.006～4 mg/L respectively.In 15 strains,11 strains showed drug-resistant to FL,8 strains to AP and FC,4 strains to KE and 1 strains to IT respectively and MIC were all >32 mg/L.Conclusion:Etest MIC were in good agreement with macrobroth MIC.The use of Etest strips for antifungial susceptibility test is a new and promising method with advantage of easy to perform, exact results and satisfactory reproducibility. Etest is valuable on clinical practice.
