Fugene 6——a new approach to induce the gene transfection in high efficiency for eukaryotic cells in vitro
10.3969/j.issn.1000-8861.2001.02.019
- VernacularTitle:Fugene 6——一种介导体外真核细胞高效率基因转染的新方法
- Author:
Yide HU
;
Nan GAO
;
Xiaoyun CAO
;
Jue ZHOU
;
Shilong CAO
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):138-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method to induce the gene transfection in high efficiency for eukaryotic cells in vitro. Methods Four kinds of p14ARF gene primarily deleted human carcinoma cell line including H460,A549,U251,and PC-3 were transfected with the human p14ARF expression vector (pCI-neo-p14ARF) by using the new nonliposomal transfection reagent Fugene 6. The efficiency of gene transfer was determined by screening the cells in G418. Results After 21 days' selection, G418-resistant clones were shown in all the transfected plate. PCR product of p14ARF gene was positive in all the G418-resistant clones. Cytotoxicity of Fugene 6 was detected. The cell proliferation activity was not affected when it was cultured in a high dose of Fugene 6. Conclusion These results demonstrate that Fugene 6 is a rapid, feasible, reproducible, and noncytotoxic gene transfection approach for eukaryotic expression vector in vitro.
