Effects of heparin on expression of collagen mRNA in rat lung fibroblast
10.3969/j.issn.1001-7399.2001.02.017
- VernacularTitle:肝素对鼠肺成纤维细胞胶原mRNA表达的影响
- Author:
Hongling YIN
;
Lianghua BIN
;
Zhongfei QIAN
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical and Experimental Pathology
2001;(2):149-151
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study the effect of heparin on expression of type collagen Ⅰ,ⅢmRNA in cultured fetal rat lung fibroblast (LFb). Methods By using Northern blot method and immage analysis,the expression of collagenⅠ, Ⅲ mRNA were quantitatively analysed in the fibroblasts treated with different concentration heparin (0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L)separately. Results Heparin treatment at 0.1 mg/L,1 mg/L, 10 mg/L and did not influence the expression of collagen Ⅰ,Ⅲ mRNA，but the expression of collagen Ⅰ,Ⅲ mRNA were inhibited at 100 mg/L. Conclusion Expression of collagen Ⅰ, ⅢmRNA is related to the concentration of heparin.
