Molecular cloning and sequencing of the cDNA of the soluble human leukocyte antigen-G1
10.3969/j.issn.1000-8861.2001.02.001
- VernacularTitle:可溶性人类白细胞抗原-G1 cDNA的克隆及序列分析
- Author:
Chongyun FANG
;
Xiongwen WU
;
Feili GONG
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a recombinant plasmid pcDNA3-sHLA-G1 expressing soluble HLA-G1. Methods Total cell RNA was extracted from the cell line Jeg-3 and the cDNA was amplified by RT-PCR； The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3-sHLA-G1 had been constructed successfully. Conclusion In this study, the recombinant plasmid pcDNA3-sHLA-G1 had been constructed successfully.
