Recombination and expression of a component of urease B subunit transmembrane protein of helicobacter pylori
10.3969/j.issn.1000-8861.2001.02.005
- VernacularTitle:大肠杆菌表达人幽门螺杆菌尿素酶B亚单位跨膜区成份
- Author:
Xuhu MAO
;
Dongshui LU
;
Hong GUO
;
Chao WU
;
Fukun WANG
;
Quanming ZOU
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):94-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To recombine and express a component of urease B subunit transmembrane protein of helicobacter pylori. Methods A 732 bp gene fragment of urease B subunit of helicobacter pylori was cloned into pET11C and transformed into BL21（DE3）E.coli. The positive clone was induced with IPTG. The expression of target protein was analysed by SDS-PAGE and Western blot. Results It is successful to construct the recombinant plasmid pET-UreB0.7 containing urease B subunit 0.7 kb gene fragment. A protein （MW≈28 000 u） with immunoreactivity， was expressed by 19.8％ in BL21（DE3）E.coli induced with IPTG. Conclusions The recombinant component of urease B subunit transmembrane protein may play a role in the research of its biological function and might be used as the vaccine against helicobacter pylori.
