Analysis of the expression of MAGE-1 gene in human esophageal carcinoma and cloning of MAGE-1 cDNA from the carcinoma
10.3969/j.issn.1000-8861.2001.02.006
- VernacularTitle:MAGE-1基因在人食管癌中的表达及基因克隆
- Author:
Liangping PENG
;
Guiqi WANG
;
Yuliang RAN
;
Haiyan LIU
;
Lixin SUN
;
Long YU
;
Zhihua YANG
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):97-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression of MAGE-1 gene in esophageal carcinoma and determine whether esophageal carcinoma patients should be eligible for MAGE-1 antigen-based vaccine therapies. Methods MAGE-1 mRNA expression in esophageal carcinoma was assessed by reverse transcription and polymerase chain reaction amplification. The PCR products were then digested by restriction endonucleases and inserted into the pET-30a(+) vector. The sequence of the inserted gene fragment was confirmed by DNA sequence analysis. Results Out of the 30 esophageal carcinomas studied, 43% of the esophageal carcinomas tissues expressed MAGE-1 gene and no expression was found in matched adjacent normal esophageal mucosae. The entire cDNA of the gene was cloned. Conclusion Owing to the high frequency of MAGE-1 gene expression in esophageal carcinoma and MAGE-1 antigen can be recognized by cytolytic T lymphocytes when presented by class-I HLA molecular, a large proportion of these patients might be suitable candidates for immune therapy involving tumor specific antigens encoded by MAGE-1 gene.