Analysis of the expression of MAGE-1 gene in human esophageal carcinoma and cloning of MAGE-1 cDNA from the carcinoma

VernacularTitle:MAGE-1基因在人食管癌中的表达及基因克隆
Author: Liangping PENG ; Guiqi WANG ; Yuliang RAN ; Haiyan LIU ; Lixin SUN ; Long YU ; Zhihua YANG
Publication Type:Journal Article
From: Immunological Journal 2001;(2):97-99
CountryChina
Language:Chinese
Abstract: Objective　To evaluate the expression of MAGE-1 gene in esophageal carcinoma and determine whether esophageal carcinoma patients should be eligible for MAGE-1 antigen-based vaccine therapies． Methods　 MAGE-1 mRNA expression in esophageal carcinoma was assessed by reverse transcription and polymerase chain reaction amplification． The PCR products were then digested by restriction endonucleases and inserted into the pET-30a(+) vector． The sequence of the inserted gene fragment was confirmed by DNA sequence analysis． Results　Out of the 30 esophageal carcinomas studied, 43% of the esophageal carcinomas tissues expressed MAGE-1 gene and no expression was found in matched adjacent normal esophageal mucosae． The entire cDNA of the gene was cloned． Conclusion　Owing to the high frequency of MAGE-1 gene expression in esophageal carcinoma and MAGE-1 antigen can be recognized by cytolytic T lymphocytes when presented by class-I HLA molecular, a large proportion of these patients might be suitable candidates for immune therapy involving tumor specific antigens encoded by MAGE-1 gene．