Study on ConA promoting phagocytosis, cytotoxicity and producing effectors of macrophages

VernacularTitle:ConA增强MΦ吞噬功能、杀瘤效应及产生效应分子的研究
Author: Lifen GAO ; Junyan LIU ; Hongming TANG ; Xueju QU ; Zhengming JIANG
Publication Type:Journal Article
From: Immunological Journal 2001;(2):110-112
CountryChina
Language:Chinese
Abstract: Objective　To study　how　ConA actives macrophages in vivo to produce cytotoxic effectors and its phagocytic functions,and　 cytotoxicity. Methods　 ConA was intraperitoneally injected(ip). Cock red blood cells(cRBC) were used to evaluate MΦ phagocytic activity,and S180 cells as target cells to analyze MΦ dependent cytotoxicity(MTC).Nitric oxide(NO),TNF-α　and IL-1 levels of MΦ cultural supernatant were measured using griess reagent,L929 cells MTT method and thymocytes proliferation test respectively. Results　 ConA could promote MΦ　to phagocytize cRBC and kill S180 cells,enhance the production of such factors as NO,TNF-α　and IL-1 by MΦ. There was significant difference compared with PBS　control group(P＜0.01). Conclusions　 ConA could stimulate MΦ to produce effectors, which mediate immune regulation of ConA to MΦ.