Insulin facilitates glucose transporter 4 gene expression in canine heart in vivo
10.3969/j.issn.1000-8861.2001.02.017
- VernacularTitle:胰岛素促进犬在体心肌细胞葡萄糖转运子4基因表达
- Author:
Renfu YIN
;
Jinming CHEN
;
Zonggui WU
;
Shaohua QIU
;
Yongmei WANG
;
Ruimei WU
;
Xiantao KONG
- Publication Type:Journal Article
- From:
Immunological Journal
2001;(2):132-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism for that insulin facilitates increase of glucose uptake. Methods The expression of myocardial GLUT4 polypeptide was determined by semiquantitative immunoblotting. The expression of GLUT4 mRNA was determined by semiquantitative Northern blotting. Results After infusing insulin for 8 hours，the expression of GLUT4 mRNA and GLUT4 polypeptide was significantly higher in canine myocardium than in those found normal ones. The glucose uptake was upregulated at the same time．Conclusions Our findings suggest that insulin facilitates the expression of GLUT4 mRNA and GLUT4 polypeptide in canine hearts. Enhanced GLUT4 expression is one of the important molecular mechanism by which myocardial cells enhance glucose uptake by insulin stimulation.
