Study on the determination of metronidazole and amoxicillin in Bijiaxilin tablet by HPLC
- VernacularTitle:铋甲西林片的高效液相色谱分析研究
- Author:
Yingxiang DU
- Publication Type:Journal Article
- From:
Chinese Pharmaceutical Journal
2001;(2):115-118
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVE In order to control the quality of Bijiaxilin tablet, a method for the determination of metronidazole and amoxicillin was developed.METHOD A HPLC procedure was established.A 6.0 mm×150 mm Shim-Pack CLC-ODS column with a mobile phase consisting of pH6.5,0.02 mol*L-1 phosphate buffer-acetonitrile(90∶10) was used.A 10 μL portion of the sample solution was chromatographed and detected at 272 nm.The effects of the types of the mobile phase on the retention behaviour of metronidazole,amoxicillin and the other components in the samples were investigated.The effect of the solvent used for sample preparation on the stability of metronidazole and amoxicillin was also studied.RESULTS Metronidazole,amoxicillin and the other components in the samples were separated completely.The recoveries were 99.88% for metronidazole and 99.96% for amoxicillin with RSD less than 1.7%.CONCLUSION The proposed method is selective,simple,accurate and precise.
