High-performance liquid chromatographic determination of floxuridine in human serum
- VernacularTitle:人血清中氟尿苷的HPLC测定方法
- Author:
Peng WANG
;
Zhu ZHU
;
Qiang FU
;
Min YE
;
Dakui LI
- Publication Type:Journal Article
- From:
Chinese Pharmaceutical Journal
2001;(2):118-120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop an HPLC method for the determination of floxuridine in human serum.METHODS With metronidazole as the internal standard,200 μL of serum was extracted by n-propyl alcohol/methyl t-butyl ether in a two-step extraction.The organic layer was evaporated under nitrogen stream and the residual was reconstituted with the mobile plase.A Shim-Pack CLC-ODS column was selected and the mobile phase was consisted of acetonitrile-phosphate buffer-water (75∶100∶900) at a flow rate of 0.6 mL*min-1.The detection wavelength was 268 nm.RESULTS A linearitywas obtained from 0.005 to 0.5 mg*L-1 of floxuridine in serum with a good correlation coefficient (r=0.9999,n=8).The intra-run and inter-run coefficients of variation were less than 4.09%.The mean recoveries were 103.00%,107.00% and 100.88% for the low,middle and high concentrations of check samples,respectively.The limit of detection was 0.001 mg*L-1.CONCLUSION The method was sensitive,specific and simple.It is suitable for clinical pharmacokinetic study.
