STUDIES OF APOPTOSIS INDUCED IN MALIGNANT LYMPHOMA CELL LINES BY ARSENIC TRIOXIDE
10.3781/j.issn.1000-7431.2001.02.001
- VernacularTitle:三氧化二砷对恶性淋巴瘤细胞株凋亡的研究
- Author:
Lin NIE
;
Yuan ZHANG
- Publication Type:Journal Article
- From:
Tumor
2001;(2):79-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of a wide range of concentrations of As2O3 malignant lymphoma cell lines. Methods Cell apoptosis situation ,cellular DNA contents and bcl-2 protein expression were determined by cell stain, TdT-mediated dUTP Nick-end Labeling (TUNEL) and flow cytometry. Results 0.5 μmol/L～2.0 μmol/L As2O3 could inhibit Raji cell growth accompanied by the appearance of morphologic characteristics of apoptosis. Number of sub-G1 cells significantly increased and bcl-2 protein expression in Raji cells was significantly down-regulated assayed by flow cytometry. The relationship between bcl-2 protein and the concentrations of As2O3 showed a dose-and time-dependent effect; so did the number of sub-G1 cells. However, there was no effect of 0.5 μmol/L～4.0 μmol/L As2O3 on Jurkat cell.Conclusion It is found that As2O3 could significantly inhibit the growth of B lymphoma cell lines and induce their apoptosis.
