EFFECTS AND CLINICAL SIGNIFICANCE OF PROGLUMIDE IN GASTRIN-STIMULATING CELL PROLIFERATION ON HUMAN COLON CELL LINE SW480
10.3781/j.issn.1000-7431.2001.02.004
- VernacularTitle:丙谷胺抑制人结肠癌细胞株SW480增殖的机制研究
- Author:
Bin XIE
;
Shuangwu HE
;
Daike WANG
- Publication Type:Journal Article
- From:
Tumor
2001;(2):88-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and clinical significance of proglumide (PGL) in gastrin-stimulating cell proliferation on human colon cell line SW480.Methods Effects of proglumide on the viable cell count (A value), IP3 (CPM value), ［Ca2＋］i and PKC activity of human colon cell line SW480 were evaluated in vitro by MTT assay, 3H-myo-inositol incorporation, fluorescence measurements and γ-32P-ATP incorporation. Results The results showed that A value of PGL＋PG group is lower than that of PG or control group (P＜0.01 in both groups); The concentration of IP3 or ［Ca2＋］i in PGL＋PG group is lower than that in PG group (P＜0.05). Conclusion These results indicate effects of proglumide may be through the phosphoinositide signalling pathway on gastrin-stimulating cell proliferation in human colon cell line SW480. It will provide an experimental evidence for antisingnal transduction therapy for patients with colon cancer.
