Hereditary nonpolyposis colorectal cancer:A genetic study
10.3969/j.issn.1001-7399.2001.01.009
- VernacularTitle:遗传性非息肉病性结直肠癌的分子生物学研究
- Author:
Shuo LIU
;
Encong GONG
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical and Experimental Pathology
2001;(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the common mutation patterns of hereditary nonpolyposis colorectal cancer(HNPCC) genes.MethodsUsing PCR-agarose electrophoresis and PCR-SSCP to screen the hMSH2, hMLH1 genes mutation of 9 HNPCC patients and 4 family members. ResultsAbnormal shifting of PCR productions was identified in 7 patients and 2 HNPCC family members out of 9 different families. ConclusionPCR-agarose and PCR-SSCP are simple, rapid , sensitive, economic and specific methods that can be used to detect HNPCC gene hMSH2, hMLH1 mutations.
