Expression of transforming growth factor β1 and platelet-derived growth factor in alveolar type Ⅱ cells
10.3969/j.issn.1001-7399.2001.01.016
- VernacularTitle:肺泡Ⅱ型TGFβ1和PDGF基因表达及其在肺纤维化中的意义
- Author:
Qingfu ZENG
;
Yong ZHAO
;
Zhongfei QIAN
;
Haiying JIANG
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical and Experimental Pathology
2001;(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
To study the expression of transforming growth factor β1 (TGFβ1) and platelet-derived growth factor (PDGF) in alveolar type Ⅱ cells and their significance in the process of pulmonary fibrosis. MethodsCultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established. In situ hybridization and irnmunohistochemieal staining were applied to detect the gene expression of TGFβ1 and PDGF-B in the alveolar type Ⅱ cells of primary culture and silicotic tissue. Results(1) In situ hybridization and immunohistochemical staining showed the positive expression of TGFβi, PDGF-B rnRNAs and their relevant proteins in cultured alveolar type Ⅱ cells. (2) The positive expression of TGFβ1, PDGF-B rnRNAs and their proteins were prominent in the proliferative alveolar type Ⅱ cells of experimental group of silicotic models. Weak expression of TGFβ1 mRNA were found only in a part of normal alveolar type Ⅱ cells of control group. ConclusionThe proliferative alveolar type Ⅱ cells express TGFβ1 and PDGF-B, which may play an important role in silicotic fibrcsis.
