Detection of trace glutaraldehyde in the fluid from venous circuit tube by HPLC
- VernacularTitle:血透患者上机前静脉管路端液体中微量戊二醛的检测
- Author:
Feifan LIU
;
Xiaoping LI
;
Lihua ZHANG
;
Tianbiao LAN
;
Siheng GUO
;
Yingqun WANG
- Publication Type:Journal Article
- From:
Chinese Pharmaceutical Journal
2001;(1):57-59
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To detect the trace glutaraldehyde in the fluid from venous circuit tube of hemodialysis,a HPLC assay was developed.METHODS 20 mL fluid taken from the venous circuit tube were derived with DNPH for 3 h,then filtered and injected. Chromatography was conducted on C18 column at 29℃.The mobile phase was consisted of 60% CH3N and 40% H3PO4 with the flow rate at 1 mL*min-1, and the detetion wavelength was at 365 nm.RESULTS The concentrations of glutaraldehyde in the fluid ranged from 14.99 to 37.40 μg.mL-1.CONCLUSION This HPLC method is simple and accurate to detect the trace glutaraldehyde remained in the fluid from venous circuit tube.
