STUDY OF CHEMOTHERAPY SENSITIVITY IN TUMOR CELL LINES ASSESSED BY APOPTOSIS
10.3781/j.issn.1000-7431.2001.01.007
- VernacularTitle:细胞凋亡检测用于肿瘤细胞株对化疗敏感性的研究
- Author:
Tonghai XING
;
Zhihai PENG
;
Guoqiang QIU
- Publication Type:Journal Article
- From:
Tumor
2001;(1):20-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study chemotherapy sensitivity and apoptosis in tumor cell line.Methods Two different human colon carcinoma cell lines, LoVo and Ls-174-t were incubated with DDP,MMC,5-FU，EPI at various peak plasma concentrations (PPC), 1/10PPC，1/5PPC，5PPC and 10 PPC. Apoptosis was detected by FACScan and TUNEL technique after 24 hours and 48 hous. Cell growth inhibition rates were assessed by MTT, DNA ladder were examined by agarose electrophoresis.Results At 48 hours, the highest cellular apoptosis rates were observed with PPC which was assessed by FACScan technique. There was a positive linear correlation between apoptosis assessed by TUNEL technique and growth inhibition rates of cells determined by MMT(P＜0.05).Conclusion Using Annexin-V-Fluos staining FACScan technique to assess apoptosis of tumor cells is an exploring and useful method which can be used to choose both kinds and doses of chemotherapeutic drugs.
