EXOGENOUS CERAMIDE-INDUCED APOPTOSIS IN COLON CARCINOMA LoVo CELLS
10.3781/j.issn.1000-7431.2001.01.009
- VernacularTitle:神经酰胺诱导大肠癌LoVo细胞的凋亡
- Author:
Xiaohua TAN
;
Yali ZHANG
;
Dianyuan ZHOU
- Publication Type:Journal Article
- From:
Tumor
2001;(1):26-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate exogenous ceramide-induced apoptosis in colon carcinoma LoVo cells.Methods LoVo cells were pretreated for 3 h with or without the presence of phorbol 12-myristate 13-acetate (PMA), a potent protein kinase C (PKC) stimulator, later treated with C2-, C6-ceramide or C2-dihydroceramide, and then passed through gel electrophoresis, Heochest 33342 fluorescence staining and flow cytometry with Annexin V/PI staining. The treated LoVo cells were observed for biochemical and morphologic changes. Results Treatment with C2- or C6-ceramide in the range of indicated concentrations (10～25 μmol/L) for 12 to 24 hours resulted in apoptosis in LoVo cells, whereas C2-dihydroceramide, which is similar to C2-ceramide in configuration but lacks the trans double bond at C4-C5 of the sphingoid base backbone, did not induce the apoptosis at the same or even higher concentrations, indicating that the ceramide-induced apoptosis was stereospecific. Moreover, the exogenous ceramide-induced apoptosis in LoVo cells was inhibited in part by PMA. Conclusion Ceramide takes part in the process of apoptotic signal transduction in LoVo cells. PKC may be one of downstream target molecules acted by ceramide.
