INDUCTION OF APOPTOSIS IN HUMAN BLANDDER CANCER CELL HTB9 AND REGULATION OF APOPTOSIS RELATED GENE EXPRESSION BY WILD-TYPE p53 GENE
10.3781/j.issn.1000-7431.2001.01.010
- VernacularTitle:p53基因诱导膀胱癌细胞HTB9凋亡及相关基因的表达
- Author:
Xiaotian ZHANG
;
Weigang CHEN
;
Jian LI
- Publication Type:Journal Article
- From:
Tumor
2001;(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the induction of apoptosis and the regulation of apoptosis related genes bcl-2, bax and ICE expression by wild-type p53 gene. Methods A p53 gene recombinant adenovirus vector, ad CMV p53, was transfected into human bladder cancer cell HTB9. The level of bax mRNA was quantified by reverse transcription-polymerase chain reaction (RT-PCR). The content of Bcl-2, Bax and ICE protein were detected by immunochemical staining. Agarose gel electrophoresis of DNA、 terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and flow cytometric technique were used for analysis of apoptosis. Results Introduction of wild-type p53 could increase the mRNA and protein level of bax, and could induce apoptosis in HTB9 cells with the apoptotic percentage of 50.4%. At the same time, the introduction also increased the protein level of ICE, but that of Bcl-2 decreased.Conclusion It's very possible that wild-type p53 gene induced apoptosis by regulating the expression of apoptosis-related genes including bax, bcl-2 and ICE.
