A study on biological characteristics of normal adult human nasal septal chondrocytes cultured in vitro
10.3969/j.issn.1001-1781.2000.11.002
- VernacularTitle:培养状态下成人鼻中隔软骨细胞生物学特性的研究
- Author:
Xuesheng LI
;
Jinzhong HUANG
- Publication Type:Journal Article
- Keywords:
Nasal septal Chondrocytes Cell culture Cytobiology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2000;(11):485-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the characteristics of normal adult human nasal chondrocytes cultured in vit-ro. Method :The adult human nasal septal chondrocytes were cultured in vitro. The changes of cellular morpholo-gy from primary to subculture were investigated ;to observe the proliferation of chondrocytes by the growth curveand the survive rate of chondrocytes;and to determine the biologic synthesis of the chondrocytes by toluidineblue,alkaline phosphatase staining and type Ⅱ collagen immunohistochemistry reaction. The survive rate of thenasal septal chondrocytes was observed after being frozen (－196C). Result: The morphology of the primarychondrocytes maintained the spherical shape ,and some became polygonal shape after 4 days. Most of the chondro-cytes transformed into the shape of the fibroblast cell since the fifth generation. The number of the chondrocytes(2th generation)after being cultured in vitro for 5 days was approximately 4 fold of that of the plating. The phe-notype of the chondrocytes was maintained in the first 4 generation,the survive rate of the chondrocytes suscitat-ing from being frozen (－196C ) was 93 %. Conclusion:The normal adult nasal septal chondrocytes cultured inmonolayer in vitro maintained the specific chondrocytes phenotype in the first 4 generation,the growth was nor-mal ,and could survive in the condition of deep-frozen.
